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#Spot color separation has 65 black in it free
New processes for detachment of larger magnetic particles from isolated cells enable use of free cells for in vivo applications. The separation process can be relatively easily scaled up and thus large amount of cells can be isolated. Many ready-to-use products are available and the basic equipment for standard work is relatively inexpensive. Magnetic separation of cells is a simple, rapid, specific and relatively inexpensive procedure, which enables the target cells to be isolated directly from crude samples containing a large amount of nontarget cells or cell fragments. Šafaříková, in Encyclopedia of Separation Science, 2000 Future Developments In addition to target protein purification, they can also be used to immobilize a target protein which then acts as a bait to pull down its interaction partner(s) from a complex biological mixture. Paramagnetic particles are available as unmodified, modified with common affinity ligands (e.g., streptavidin, GSH, Protein A, etc.), and conjugated particles with specific recognition groups such as monoclonal and polyclonal antibodies ( Koneracka et al., 2006). The capability of miniaturization and parallel screening of multiple conditions, such as growth conditions for optimal protein expression and buffer conditions for purification, makes magnetic separation amenable to high-throughput analysis which can significantly shorten the purification process ( Saiyed et al., 2003). The method can be used on viscous materials that will otherwise clog traditional columns and can therefore simplify the purification process by eliminating sample pretreatment, such as centrifugation or filtration to remove insoluble materials and particulates. Magnetic separation can significantly shorten the purification process by quick retrieval of affinity beads at each step (e.g., binding, wash, and elution), and reduce sample dilution usually associated with traditional column-based elution. Kate Zhao, in Methods in Enzymology, 2009 2.4 Magnetic affinity beads